Genomes of Golden-winged and Blue-winged Warblers
Look to be 99.97% Alike
Golden-winged Warbler by Tom Johnson, Blue-winged Warbler by Robert Dorman

Genomes of Golden-Winged and Blue-Winged Warblers
Look to be 99.97% Alike

New research findings could have implications for conservation
of one of America’s fastest declining songbirds
 

 
For release: August 18, 2016

Ithaca, N.Y.—For decades, conservationists have considered Blue-winged Warblers to be a threat to Golden-winged Warblers, a species being considered for federal Endangered Species listing with populations that have declined 66 percent since 1968, according to the North Ameri­can Breeding Bird Survey.

The two species are known to frequently interbreed where they co-occur, and scientists have been concerned that the more numerous Blue-winged Warblers would geneti­cally swamp the rarer golden-wing gene pool.

New research from the Cornell Lab of Ornithology’s Fuller Evolutionary Biology Program shows that the genetic differences between Golden-winged and Blue-winged Warblers are found in just six regions (or .03 percent) of their entire genomes. One of those regions contains genes that control throat coloration. Illustrations by Liz Clayton Fuller, Bartels Science Illustration Intern.
New research from the Cornell Lab of Ornithology’s Fuller Evolutionary Biology Program shows that the genetic differences between Golden-winged and Blue-winged Warblers are found in just six regions (or .03 percent) of their entire genomes. One of those regions contains genes that control throat coloration. ‚ÄčIllustrations by Liz Clayton Fuller, Bartels Science Illustration Intern.
Click image for high-resolution version.

New research out of the Cor­nell Lab of Ornithology’s Fuller Evo­lutionary Biology Program shows that, genetically speaking, Blue-winged and Golden-winged warblers are almost identical. The scientists behind the research say that the main differences between the two species are in feather color and pattern, in some cases just a simple matter of dominant or recessive pairings of alleles. 

“We think we have finally pinpoint­ed the proverbial genomic ‘needle in the haystack’ between these taxa,” says study co-author Da­vid Toews, who adds that the findings suggest conservationists should be less concerned with hybridization and primarily focused on preserving habitat for both species. “This is something that conser­vation practitioners have wanted for a very long time.”
 
The research is published in the September issue of the journal Current Biology by Toews and his fellow Cornell Lab postdoctoral researcher Scott Taylor, along with partners from Cornell University’s Department of Biological Statistics and Computational Biology, the University of California at Riverside, and Environment and Climate Change Canada.
 
The team investigated the genetic architecture behind the differences between the two warblers by analyzing the genomes of 10 Golden-winged Warblers and 10 Blue-winged Warblers from New York, with birds sampled from the Sterling Forest along the New Jersey border up to the St. Lawrence River Valley. Across their analysis of the entire genomes of both species, they found only six regions (or less than .03 percent) that showed strong differences. In other words, Blue-winged and Golden-winged warblers are 99.97 percent alike genetically.
 
One of the differentiating regions has a gene that likely controls yellow/white versus black throat coloration; the black throat of the Golden-winged Warbler is a classic Mendellian recessive trait, occurring only in birds that have a pair of recessive alleles of this genetic variant. Another region likely controls body color; the yellow body of Blue-winged Warblers is likely an incompletely recessive trait.
 
When Blue-winged and Golden-winged warblers interbreed, they produce various hybrids, including two forms called the Brewster’s Warbler (with a light body and no black throat) and Lawrence’s Warbler (with a yellow body and black throat). This new research shows that the “Brewster’s” form of Golden- and Blue-winged warbler hybrids seems to be an expression of dominant traits for throat and body color, whereas the “Lawrence’s” form of hybrid exhibits recessive trait expression for both.
 
The research supports a model proposed by John T. Nichols of the American Museum of Natural His­tory back in 1908 that the Brewster’s form of Golden- and Blue-winged war­bler hybrids is an expression of domi­nant traits, and the Lawrence’s form is a recessive trait expression. Put another way, the striking visu­al differences between Golden- and Blue-winged warblers could be con­sidered akin to the differences between humans with and without freckles. The research also shows that golden-wings and blue-wings have even less genet­ic differentiation than two subspecies of the Swainson’s Thrush, the Ol­ive-backed and Russet-backed forms.
 
Toews, Taylor, and their collaborators also analyzed a subset of genetic regions from Golden- and Blue-winged warblers from across their range and found that individuals from as far away as Manitoba and Missouri show similarly little genomic difference. This kind of genetic data carries information not only on how different the birds are today, but also on how long they might have been separated—or interbreeding—in the past.
 
Going back 250 years to when Golden-winged and Blue-winged warblers were first scientifically described, they were known to live in different places, with golden-wings in the Northeast and upper Midwest and blue-wings in a band slightly farther south from the Ozarks to the Appala­chian Mountains. And it was thought that forest clearing by European settlers starting in the late 1700s caused the habitat chang­es that brought the two species togeth­er, thereby causing their hybridization.
 
In using the whole genome to look deeper into these birds’ evolutionary histories, Taylor and Toews made a surprising discovery—it turns out that these two species have probably been intermixing, at least intermittently, for thousands of years, well before Euro­peans colonized North America.
 
“This hybridization has been con­sidered our [humans’] fault,” said Tay­lor. “But the propensity for these two species to hybridize is natural and ap­pears to be part of their pre-European evolutionary history.”
 
Hybridization was one of the threats identified in the national Gold­en-winged Warbler Conservation Plan, published by a consortium of conser­vation groups and government agen­cies in 2012, though loss of young forest habitat was cited as the primary threat and most of the work driven by the plan has benefited both golden-wings and blue-wings. Hybridization, and the question of human causation, may play a role in the U.S. Fish and Wildlife Service’s upcoming decision on wheth­er Golden-winged Warblers warrant federal protection under the Endan­gered Species Act.
 
The matter of golden-wing/blue-wing genetic similarity may pose a tricky question for the American Ornithologists’ Union, too, should the AOU’s North American Classification Committee be asked to consider this evidence in a proposal to lump golden-wings and blue-wings into a single species.
 
“Someone could definitely make a case for Golden-winged Warblers and Blue-winged Warblers being one polymorphic species. They are extremely similar,” Taylor says. “From a conservation standpoint, they need comparable types of young forest habitat so we could treat them similarly, whether it’s in the name of conserving one species or a complex of two closely-related species.”

 
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Images:
Click on links in the captions to download high-resolution versions of the images in this release. These images are freely available for use in stories provided that you use the supplied photo credit.
 
 
Contact:
Pat Leonard, Cornell Lab of Ornithology, (607) 254-2137, pel27@cornell.edu
The Cornell Lab of Ornithology is a membership institution dedicated to interpreting and conserving the earth’s biological diversity through research, education, and citizen science focused on birds. Visit the Cornell Lab’s website at http://www.birds.cornell.edu.

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