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Target validation of Pim-2 kinase in a hepatic cancer cell HepG2: HepG2 cells were grown in 2D and the target gene quantitatively silenced by means of viral gene transfer prior to 3D microtissue formation. Within one week a significant 42% reduction of the microtissue area was observed in Pim-2-silenced HepG2 cells compared to the control microtissues transduced with a non-target shRNA. This result suggests the implication of Pim-2 kinase activity in hepatic cancer growth.
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Genetic modification of 3D cancer co-cultures: SIRION BIOTECH used lentiviral TeT-inducable gene expression for HCT116 modification. Cells were grown under Dox-free conditions for microtissue formation showing that target gene effects did not prevent 3D microtissue formation.
For more details about these exciting studies, please take a look at the white paper published jointly by SIRION and InSphero.
Source: SIRION BIOTECH GmbH
Opportunities to meet the InSphero team at 8th World Congress on Alternatives and Animal Use, Drug Discovery 11 & MipTec 2011
Visit the InSphero booth and presentations at these events to learn how organotypic 3D microtissues are bridging the gap between non-physiological 2D cell culture and expensive animal models for drug efficacy and safety testing:
8th World Congress on Alternatives and Animal Use in the Life Sciences, August 21-25, Montreal, Canada
Our organotypic 3D microtissues are helping to replace and reduce animal testing.
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Oral presentation “A novel platform for automated production and screening of scaffold-free, organotypic microtissues ”, please check overall program on WC8 website for the date and time
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Poster presentation “Towards Automation of the Embryonic Stem Cell Test”, display on Tuesday, August 23 from 7:00 AM to 3:15 PM
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Poster presentation “An Organotypic Microliver Platform for High Throughput Drug Testing”, display on Wednesday, August 24 from 7:00 AM to 3:15 PM
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Learn more here.
Drug Discovery’11, September 7-8, Manchester, UK
Meet with us to discuss exciting new liver data supporting the use of 3D microtissue models.
Learn more here.
MipTec, September 20-22, Basel, Switzerland
Our full team of experts will be on hand to advise on all aspects of 3D cell culture.
Learn more here.
To arrange a meeting or a webinar please send us an E-mail.
Invitation to InSphero workshop “Compound de-risking with 3D-cell-culture systems”, Sept 9th in Manchester, UK - with 50% discount for Drug Discovery 2011 participants!
Attending an InSphero workshop is an excellent way to get fully updated with the latest information on 3D-cell-culture technologies for toxicological and efficacy studies. Our expert speakers, with their many years of combined experience, are in a unique position to provide you with information on different approaches to 3D culture and assays and the main issues affecting quality and costs in this segment of the industry today. The next half-day workshop will take place in Manchester, UK, on September 9th, 2011, starting at 8:30am. Places are limited, so please register as soon as possible here.
*Special offer for Drug Discovery 2011 participants: visit the InSphero stand (D6) at Drug Discovery 2011 to request a 50% discount voucher for this workshop!*
InSphero and INTEGRA cooperate
InSphero and Integra Biosciences, a Swiss-based supplier of high-quality liquid handling tools for laboratory use, will cooperate on providing high-performance liquid-handling solutions for 3D cell culture. InSphero scientists and technicians have extensively tested Integra’s VIAFLO electronic pipetting systems which have provided thoroughly satisfactory results with InSphero's GravityPLUS™ culture plates. The VIAFLO precision flow control capability is particularly helpful for microtissue-based assays. We're confident that the complementary skills and know-how of both companies will facilitate industry-wide implementation of 3D microtissues for drug testing.
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